Haematoxylin counterstaining of immunofluorescence preparations.

Haematoxylin counterstaining of immunofluorescence preparations.

Improved mountant for immunofluorescence preparations.

References: Kaufman, G. L, Nester, J. F., and Wasserman, D. E. (1971). An experimental study of lasers as excitation sources for automated fluorescent antibody instrumentation. J. Histochem. Cytochem., 19, 469-476. Koch, K. F. (1972). Fluorescence Microscopy; Instruments, Methods, Applications. Leitz, Wetzlar. Rygaard, J., and Olsen, W. (1971). Determination of characteristics of interference f...

Haematoxylin bodies in Hodgkin's disease.

A case is described in which haematoxylin bodies were found in a lymph node altered by Hodgkin's disease. They can no longer therefore be regarded as entirely specific for lupus erythematosus.

Immunofluorescence Pattern of Autoimmune Bullous Diseases in Iranian Patients

Background & Objective: Autoimmune bullous diseases are associated with autoimmunity against structural components in the skin and mucous membranes. Autoantibodies are against the intercellular junctions in pemphigus disease and hemidesmosomal unchoring complex in pemphigiod diseases and epidermolysis bullosa aquisita. The tissue-bound and circulating serum autoantibodies can be detect...

Immunofluorescence Method for The Detection of Non-Pathogenic Influenza Virus

The direct F.A. Method has been used for diffe­rentiation of virulent and avirulent lines of influenza viruses. A positive fluorescence was obtained indi­cating the level of virus in the tissues, sorbing with dried mouse powder helped to reduce non-specific staining. The virulent line showed the greatest num­ber of fluorescent cells with the peak at 10 hours. the non-virulent lines showed a gra...